Malonyl-CoA was thus examined for hydrolysis catalyzed by HMWP1 under similar conditions. The relative catalytic efficiency for malonyl-CoA hydrolysis (kcat = 6.2 min −1 and Km = 3.3 mM) was 50- and ...

Malonyl-CoA, produced by the first committed step of fatty acid biosynthesis, is a precursor for many valuable bioproducts, making it an important metabolic engineering target. Here, we establish a ...

β-Ketoacyl–acyl carrier protein (β-Ketoacyl-ACP) synthase (KAS) III catalyzes the first step in fatty acid biosynthesis, involving a Claisen condensation of the acetyl-CoA starter unit with the first ...